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Antibody targeting of nanoparticles to tumor-specific receptors: immunoliposomes.
Methods Mol Biol. 2010;624:295-308
Authors: Rothdiener M, Beuttler J, Messerschmidt SK, Kontermann RE
Immunoliposomes generated by coupling of antibodies to the liposomal surface allow for an active tissue targeting, e.g., through binding to tumor cell-specific receptors. Instead of whole antibodies, single-chain Fv fragments (scFv), which represent the smallest part of an antibody containing the entire antigen-binding site, find increasing usage as targeting moiety. Here we provide protocols for the preparation of type II scFv immunoliposomes by the conventional coupling method as well as the post-insertion method. Furthermore protocols to analyze binding of these immunoliposomes to antigen-expressing cells as well as internalization through receptor-mediated endocytosis are included.
PMID: 20217604 [PubMed - indexed for MEDLINE]
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